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Anti-tumor efficacy of B7-H3-haCAR-T cells in vitro


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(a) Schematic representation of DNA structures for the construction of B7-H3-Mcherry fusion proteins; flow cytometric assay of B7-H3 from SGC-Mcherry cells and B7-H3-SGC-Mcherry cells with a FITC labeled anti-B7-H3 monoclonal antibody; (b) 51Cr-release assays of haCAR-T cells against B7-H3-SGC-Mcherry or SGC-Mcherry cell at different E:T proportions; (c) The IFN-γ and IL-2 secretion levels of haCAR-T cells co-incubated with target cells in E:T proporation of 5:1 at 8 h were measured through ELISA; (d) Representative flow cytometer dot plots displaying haCAR-T cells and tumor cells after 72 h of co-incubated; (e) Absolute numbers of haCAR-T cells and tumor cells after 72 h of co-incubated (**P<0.01, ***P<0.001)

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