Differentiation of HUMSCs into IPCs Light micrograph of HUMSCs before (a) or after 14 days (b) of the induction. The induced cells could be stained by DTZ (c) and expressions of C-peptide (d) and PP (e) were positive by immunofluorescence staining. The nuclei were counterstained using Hoechst 33342 (f). Pdx1, Oct4 and Insulin expressions were determined during the induction by real-time PCR and the maximum of gene expression was set as 1 (g). The insulin release at low (2.8 mmol/L) and high (16.7 mmol/L) levels of glucose challenge was made in HUMSCs (0 day) and after 7, 14 and 21 days of the induction (h). *P＜0.05 vs. 7 days. Scale bar: 50 μm