Prediction of miRNAs targeting Isl1 3' UTR TargetScan showed the target site of miR-128 and miR-216a was the same and conserved from position 170 to 177 (a); The target sites (CACTGTG) of two miRNAs on Isl1 3' UTR were also the same and the seed location was 170 and the target score was 78 shown by miRDB (b). RNAhybrid showed the "seed"(GUGACAC) of two miRNAs was the same and the MFE was -24.1 kcal/mol and -20.8 kcal/mol, respectively (c); 1 kcal=4.2 kJ