Identification of miR-128 or/and miR-216a regulating Isl1 expression (a) Relative luciferase activity cotransfected with empty reporter (vehicle), pmirGLO-Isl1-wt (wt) or pmirGLO-Isl1-mut (mut), and control mimic duplexes (control), miR-128 or miR-216a mimic and miR-128 or miR-216a inhibitor; Relative luciferase activity from vehicle in control group was defined as 1, *P＜0.05; (b) Real-time PCR analysis of miR-128, miR-216a and Isl1 mRNA expression levels during the induction; Gene expression in HUMSCs (0 day) was defined as 1 and the y-axis represented relative expression level compared with expression in HUMSCs