Open Access
Table 1
Primer sequence of real-time PCR
| Gene | Primer sequence | Annealing T /℃ |
|---|---|---|
| Oct4 | Forward: 5'-GGGTGGAGGAAGCTGACAAC-3' Reverse: 5'-GGTTGCCTCTCACTCGGTTC-3' |
60 |
| Isl1 | Forward: 5'-TGCAAGGACAAGAAGCGAAG-3' Reverse: 5'-GAGTTCCTGTCATCCCCTGG-3' |
62 |
| Pdx1 | Forward: 5'-CCCTCCTACAGCACTCCACC-3' Reverse: 5'-CCGCTGTGTGTGTTAGGGAG-3' |
64 |
| Insulin | Forward: 5'-CAGATCACTGTCCTTCTGCC-3' Reverse: 5'-GTTGGTTCACAAAGGCTGCG-3' |
62 |
| MafA | Forward: 5'-TCATCCGGCTCAAGCAGAAG-3' Reverse: 5'-GTTGGCACTTCTCGCTCTCC-3' |
62 |
| Gapdh | Forward: 5'-TGCACCACCAACTGCTTAGC-3' Reverse: 5'-GGCATGGACTGTGGTCATGAG-3' |
60 |
| miR-128 | Forward: 5'-TCACAGTGAACCGGTCTCTTT-3' | 62 |
| miR-216a | Forward: 5'-TCACAGTGGTCTCTGGGATTAT-3' | 62 |
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