Biology

CLC number: Q189

Anti-Acute Fatigue Effects of Ethanol Extract of Bidens pilosa L. and the Profiling of Antioxidant Index in ICR Mice

鬼针草(Bidens pilosa L.)醇提物在ICR小鼠上的抗急性运动疲劳效应及相关抗氧化指标变化

¹ School of Physical Education, Yunnan Normal University, Kunming 650500, Yunnan, China
² School of Life Sciences, Yunnan Normal University, Kunming 650500, Yunnan, China
³ School of Ecology and Environmental Science, Yunnan University, Kunming 650050, Yunnan, China

^† Corresponding author. E-mail: gw_wang@163.com (WANG Gongwu); juncao@vip.163.com (CAO Jun)

Received: 24 February 2024

Abstract

The effects of ethanol extract of Bidens pilosa L. (EEB) on acute exercise fatigue and its underlying biochemical mechanism were investigated in this study. Sixty adult male ICR mice were divided into control, model, vitamin C (VC) 100, EEB40, EEB80, and EEB160 groups, receiving VC (100 mg/kg) or EEB (40, 80, 160 mg/kg) for 28 days (intragastrically, I.G.). The mice underwent tail-suspension, elevated plus maze (EPM), rotarod, and loaded swimming tasks and biochemical indices were measured. There were no significant differences in body weight, tail suspension time, EPM open arm time/entries and serum cortisone levels among the groups. Compared with the model group, there was an increase in rotarod latency in the VC100/EEB80 groups and an increase in loaded swimming time in the EEB80/EEB160 groups. Furthermore, the haptic and muscle glycogen levels decreased in the model group, while the haptic glycogen levels increased in the all VC/EEB groups. Similarly, the serum lactate and creatinine levels increased in the model group, but decreased in lactate (except for EEB160), creatinine (except for EEB40) and lactate dehydrogenase in the EEB80 group. In the liver, malonaldehyde (MDA) and oxidized glutathione (GSSG) levels increased in the model group; however, glutathione reductase (GR) (except for EEB40), glutathione (GSH) and GSH/GSSG ratios increased, with GSSG levels decreasing in all VC/EEB groups. In the quadriceps, the GR levels increased in the model, whereas it decreased in the VC100, EEB40 and EEB80 groups. These results suggest that EEB has anti-acute fatigue effect, potentially attributed to mitigate metabolite accumulation, enhancing glycogen reserves, and fortifying the antioxidant mechanism.

摘要

本文探讨了鬼针草(Bidens pilosa L.)醇提物(EEB)的抗急性运动性疲劳作用及其生化机制。将60只成年雄性ICR小鼠分为对照组、模型组、维生素C(VC)100组、EEB40组、EEB80组和EEB160组, 分别给予VC(100 mg/kg)或EEB(40、80和160 mg/kg)灌胃28天, 检测动物在悬尾、高架十字迷宫(EPM)、转棒和力竭负重游泳任务中的行为学表现, 并检测其生化指标。结果表明, 小鼠体重、悬尾不动时间、EPM开臂时间和进入次数及血清皮质酮无组间差异; VC100、EEB80组转棒时间, EEB80、EEB160组力竭时间较模型组升高; 模型组肝糖原和肌糖原水平较对照组降低, 而VC/EEB组肝糖原水平较模型组升高; 同样, 模型组血清乳酸、肌酐水平较对照组升高, 而VC/EEB组乳酸(EEB160除外), 肌酐(EEB40除外), 和EEB80组乳酸脱氢酶水平较模型组降低。肝: 模型组丙二醛和氧化型谷胱甘肽(GSSG)水平较对照组升高, 而VC/EEB组(EEB40除外)谷胱甘肽还原酶(GR)水平及所有VC/EEB组还原型谷胱甘肽(GSH)、GSH/GSSG比值较模型组升高, 而GSSG水平较模型组降低。股四头肌: 模型组仅GR水平较对照组升高, VC/EEB组(EEB160除外)GR水平较模型组降低。结果提示, EEB具有抗急性疲劳作用, 可能与减少机体代谢产物的累积、增加糖原储备和增强抗氧化机制相关。