Open Access
Table 1
Comparison of different detection methods based on CRISPR-Cas system
| Method | Target gene | Detection time | Sensitivity | Specificity | Virus | References |
|---|---|---|---|---|---|---|
| DETECTR | N, E | 45 min | 10 copies | High | SARS-CoV-2 (NC_045512) | [9] |
| opvCRISPR | S | 45 min | 5 copies | High | SARS-CoV-2 (NC_045512) | [10] |
| AIOD-CRISPR | N | 40 min | 1.3 copies | High | SARS-CoV-2 (NC_045512) | [25] |
| SHERLOCK | ORF1ab, S | 40 min | 100 copies | High | SARS-CoV-2 (NC_045512) | [12] |
| mCARMEN | S | < 5 h | 100 copies | High | SARS-CoV-2(NC_045512.2), Omicron, Delta | [26] |
| FnCas9-based CRISPR diagnostic | S | < 1 h | Viral load (qRT-PCR Ct < 25) |
Moderate | SARS-CoV-2, SARS-CoV-2 (N501Y, E484K, T716I mutation) | [27] |
| CRISPR-Cas13-based transcription amplification | E | < 2 h | 82 copies | High | SARS-CoV-2, SARS-CoV-2 (D614G mutation), MERS,and SARS, H1N1, H7N9,and H9N2 | [28] |
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