Biology

CLC number: O614

Synthesis, Cytotoxicity, Apoptosis and Cell Cycle Arrest of a Ruthenium(II)-Substituted Keggin Polyoxotungstate

钌多取代Keggin-型多钨酸盐的合成、细胞毒性、细胞凋亡和细胞周期研究

College of Chemical Engineering and Technology, Taiyuan University of Science and Technology, Taiyuan 030024, Shanxi, China

^† Corresponding author. E-mail: yanzhang872010@163.com

Received: 15 September 2023

Abstract

The ruthenium multi-substituted polyoxotungstate, K₇[SiW₉O₃₇Ru₄(H₂O)₃Cl₃]·15H₂O (S1), was synthesized by a conventional aqueous solution containing the trilacunary Keggin-anions β-Na₉HSiW₉O₃₄·12H₂O (S2) and RuCl₃·nH₂O (S3). Compound S1 was characterized by elemental analysis, energy-dispersive X-ray spectroscopy (EDS), thermogravimetric analysis (TG), infrared spectroscopy (IR), uliraviolet visible absorption spectroscopy (UV/Vis) and X-ray photoelectron spectroscopy (XPS). The cytotoxicitycy of S1 was tested in C33A (human cervical cancer), DLD-1 (human colon cancer), HepG2 (human liver cancer) and human normal embryonic lung fibroblasts cell (MRC-5). And the viability of these treated cells was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.To explore the mode of cell death induced by S1, morphological study of DNA damage and apoptosis assays were conducted. These analyses revealed that S1 exerted its cytotoxic effect in a dose-dependent manner, primarily triggering apoptotic cell death. Cell cycle analysis by flow cytometry indicated that compound S1 caused cell cycle arrest and accumulated cells in S phase.

摘要

本文以三缺位Keggin-型阴离子β-Na₉HSiW₉O₃₄·12H₂O (S2)和RuCl₃·nH₂O (S3)为原料, 在水溶液中合成了一个钌多取代多钨酸盐, 其化学式为: K₇[SiW₉O₃₇Ru₄(H₂O)₃Cl₃]·15H₂O (S1)。通过元素分析、能谱、热重、红外、紫外和X-射线光电子能谱对化合物S1进行了表征。通过MTT法测试了化合物S1对C33A、DLD-1、HepG2三种肿瘤细胞和人正常胚肺成纤维细胞MRC-5的细胞毒性。通过观察细胞形态及流式细胞仪考察了肿瘤细胞的死亡方式。实验结果表明化合物S1诱导肿瘤细胞凋亡而非坏死, 并且细胞存活率与S1的浓度呈梯度关系。最后通过流式细胞仪分析细胞周期的变化, 结果显示化合物S1使细胞周期停留在S期。